Ribosomal ribonucleic acid synthesis in Bacillus subtilis
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چکیده
منابع مشابه
Effect of chloramphenicol on the synthesis and stability of ribonucleic acid in Bacillus subtilis.
The effect of chloramphenicol on the synthesis and accumulation of ribonucleic acid (RNA) in Bacillus subtilis was studied. In the presence of chloramphenicol, transfer RNA and ribosomal RNA were synthesized as rapidly 2 to 3 hr after challenge as they were just prior to the addition of the antibiotic. However, under the same conditions, net RNA accumulation ceased after only 30 to 45 min. The ...
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Lipiarmycin inhibited the activity of deoxyribonucleic acid-dependent ribonucleic acid (RNA) polymerase in vitro. We showed that inhibition was due to interference by lipiarmycin with the activity of sigma-containing molecules of RNA polymerase. Transcription by core enzyme was relatively resistant to the drug, but addition of sigma led to highly drug-sensitive RNA synthesis. We isolated lipiar...
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Demain, A. L. (Merck & Co., Inc., Rahway, N.J.), R. W. Burg, and D. Hendlin. Excretion and degradation of ribonucleic acid by Bacillus subtilis. J. Bacteriol. 89:640-646. 1965.-Bacillus subtilis MB-1480 was found to produce several 5'-ribonucleotides in the extracellular medium, in addition to the previously found 5'-guanosine monophosphate and guanosine diphosphate. The nucleotides accumulated...
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Ribosomes and immature ribonucleoprotein particles were isolated from extracts of log-phase cells grown under various conditions. Quantitative measurements were made to determine the relative amounts of immature particles present in the extracts. The results indicate that the steady-state level of ribosomal precursors accounted for essentially a constant fraction of the total ribonucleic acid (...
متن کاملTwo-dimensional restriction analysis of the Bacillus subtilis genome: gene purification and ribosomal ribonucleic acid gene organization.
With two-dimensional restriction enzyme analysis we have been able to cleave the Bacillus subtilis genome and resolve the resulting deoxyribonucleic acid (DNA) segments into discrete bands on agarose gels. A general procedure for gene purification has been developed by coupling multidimensional restriction analysis with a biological assay for gene detection. The organization of ribosomal ribonu...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1971
ISSN: 0306-3283
DOI: 10.1042/bj1220139